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Journal Article 12

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Antibiotic resistance 1

Antibiotic resistance genes 1

Antibiotic resistance genes (ARGs) 1

Biohydrometallurgy 1

CRISPR/Cas9 1

Cd(II) and Cr(VI) 1

Cd-tolerant bacteria 1

Chlorination 1

Community structure 1

Conjugation 1

Electrochemically active bacteria 1

Extracellular DNA/ARGs 1

Fluorescence probe 1

HindIII 1

Indigenous microorganisms 1

Indigenous plasmid 1

Microbial electrolysis cell 1

Molecular biological techniques 1

Nanofullerene 1

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Transport of antibiotic resistance plasmids in porous media and the influence of surfactants

Peipei Chen, Chaoqi Chen, Xiqing Li

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 2, doi: 10.1007/s11783-017-0986-7

Abstract: In this work, transport of an indigenous resistance plasmid pK5 in porous media was investigated throughA similarity in transport behavior was also found between pK5 and an engineered plasmid pBR322 that hasCetyltrimethyl ammonium bromide (CTAB), a cationic surfactant, was found to significantly enhance plasmidThe negligible impact of surfactants and the similarity between the transport of engineered and indigenousplasmids indicate that under environmentally relevant conditions, indigenous plasmids in soil also have

Keywords： Indigenous plasmid     Transport     Porous media     Surfactants    

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The greater roles of indigenous microorganisms in removing nitrobenzene from sediment compared with the

Xiangqun Chi, Yingying Zhang, Daosheng Wang, Feihua Wang, Wei Liang

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 1, doi: 10.1007/s11783-018-1016-0

Abstract: rapidly and was removed completely within 20 days in native sediments, raising the possibility that indigenousThe abundance of inoculated strain JS45 and indigenous bacteria in sediments was quantified using real-timeFurthermore, community structure of the indigenous bacteria was analyzed through high throughput sequencingThe results showed that indigenous bacteria in native sediments were abundant, approximately 10 CFU/Based on the results, it can be concluded that indigenous microorganisms including and can have great

Keywords： Community structure     Indigenous microorganisms     Nitrobenzene     Plant-microbe associated remediation     Sediment    

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Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 253-257 doi: 10.1007/s11684-007-0048-9

Abstract: The purpose of this work was to construct the plasmid that could direct the synthesis of siRNA-like transcriptsof the target gene were chemically synthesized and annealed and were then ligated with pSUPER EGFP1 plasmidThen the plasmids were transfected into the cells at different ratios of plasmid to Lipofectamine.After optimizing the ratio of plasmid to Lipofectamine, we achieved high transfection efficiency in HeK293

Keywords： HindIII     transfection efficiency     detection     enhanced     different    

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Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination

Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1124-5

Abstract:

Plasmid DNA/ARGs were less readily broken down

Keywords： Antibiotic resistance     Antibiotic resistance genes (ARGs)     Extracellular DNA/ARGs     Chlorination    

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Firm innovation system: Paths for enhancing corporate indigenous innovation capability

Jin CHEN, Ximing YIN, Jizhen LI

Frontiers of Engineering Management 2020, Volume 7, Issue 3,   Pages 404-412 doi: 10.1007/s42524-020-0116-2

Abstract: achieve global innovation leadership, firms in China and other emerging economies must develop strong indigenousThis study contributes to the systematic approach for enhancing corporate indigenous innovation capability

Keywords： innovation-driven strategy     firm innovation system     national innovation system     holistic innovation theory     core competence    

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Genetic diversity and population structure of indigenous chicken breeds in South China

Xunhe HUANG,Jinfeng ZHANG,Danlin HE,Xiquan ZHANG,Fusheng ZHONG,Weina LI,Qingmei ZHENG,Jiebo CHEN,Bingwang DU

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 2,   Pages 97-101 doi: 10.15302/J-FASE-2016102

Abstract: A total of 587 individuals from 12 indigenous chicken breeds from South China and two commercial breedsHigher genetic diversity was revealed in the indigenous chicken breeds rather than in the commercialPotential introgression from the commercial breeds into the indigenous chickens was also detected.The population structure of these indigenous chicken breeds could be explained by their geographicalData generated in this study will provide valuable information to the conservation for indigenous chicken

Keywords： microsatellites     genetic diversity     population structure     indigenous chicken     South China     conservation    

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: with lactoferrin promoter mutations were obtained with an efficiency of 24% (7/29) by injecting the plasmidPlasmid integration and off-targeting of pX330 were not detected.These results confirmed that pronuclear injection of a circular plasmid is a feasible and efficient method

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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Influences and mechanisms of nanofullerene on the horizontal transfer of plasmid-encoded antibiotic resistance

Qingkun Ji, Caihong Zhang, Dan Li

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 6, doi: 10.1007/s11783-020-1287-0

Abstract: Abstract • Sub-inhibitory levels of nC60 promote conjugative transfer of ARGs. • nC60 can induce ROS generation, oxidative stress and SOS response. • nC60 can increase cell membrane permeability and alter gene expression. • Results provide evidence of nC60 promoting antibiotic resistance dissemination. The spread and development of antibiotic resistance globally have led to severe public health problems. It has been shown that some non-antibiotic substances can also promote the diffusion and spread of antibiotic resistance genes (ARGs). Nanofullerene (nC60) is a type of nanomaterial widely used around the world, and some studies have discovered both the biological toxicity and environmental toxicity of nC60. In this study, cellular and molecular biology techniques were employed to investigate the influences of nC60 at sub-minimum inhibitory concentrations (sub-MICs) on the conjugation of ARGs between the E. coli strains. Compared with the control group, nC60 significantly increased the conjugation rates of ARGs by 1.32‒10.82 folds within the concentration range of 7.03‒1800 mg/L. This study further explored the mechanism of this phenomenon, finding that sub-MICs of nC60 could induce the production of reactive oxygen species (ROS), trigger SOS-response and oxidative stress, affect the expression of outer membrane proteins (OMPs) genes, increase membrane permeability, and thus promote the occurrence of conjugation. This research enriches our understanding of the environmental toxicity of nC60, raises our risk awareness toward nC60, and may promote the more rational employment of nC60 materials.

Keywords： Nanofullerene     Sub-minimum inhibitory concentrations     Antibiotic resistance genes     Conjugation     Molecular biological techniques    

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Response of indigenous Cd-tolerant electrochemically active bacteria in MECs toward exotic Cr(VI) based

Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 4, doi: 10.1007/s11783-018-1057-4

Abstract:

Cell membrane of indigenous Cd-tolerant EAB harbored more cadmium

Indigenous Cd-tolerant EAB cytoplasm located

Keywords： Microbial electrolysis cell     Electrochemically active bacteria     Cd-tolerant bacteria     Cd(II) and Cr(VI)     Fluorescence probe    

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Bioleaching of spent Ni-Cd batteries and phylogenetic analysis of an acidophilic strain in acidified sludge

ZHAO Ling, WANG Liang, YANG Dong, ZHU Nanwen

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 4,   Pages 459-465 doi: 10.1007/s11783-007-0073-6

Abstract: discarded batteries, in which sewage sludge is used as microorganisms and culture due to the presence of indigenous

Keywords： Biohydrometallurgy     discarded     indigenous     two-step continuous     presence    

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Indigenous and Integrated Innovation Driving the Boom in China's High-Speed Rail Technologies

Ministry of Science and Technology of the PRC

Engineering 2015, Volume 1, Issue 1,   Pages 9-10 doi: 10.15302/J-ENG-2015023

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Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 287-291 doi: 10.1007/s11684-009-0050-5

Abstract: In order to explore the role of cyclin-dependent kinase 4 (cdk4) in neurodegenerative diseases, lentiviral-delivered RNA interference (RNAi) was used to silence the expression of the murine cdk4 gene . Three cdk4-shRNAs of mouse and a negative sequence were designed. After synthesis and annealing, double strand oligonucleotides were cloned into a linearized pSIH1-H1-copGFP shRNA vector. It was confirmed by polymerase chain reaction (PCR) and sequencing that three pairs of cdk4-shRNAs and a negative shRNA were correctly inserted into the pSIH1-H1-copGFP vector. The above recombinants were transfected by lipofectamine into BV-2 cells. The gene silencing efficacy rates of the 3 targets were compared by Western blotting. The cdk4-siRNA2 was the most effective in silencing cdk4. The optimized pSIH1-cdk4-siRNA2 and pSIH-negative-siRNA were co-transfected into 293T cells with the lentiviral packaging plasmids respectively. The culture supernatant was harvested and condensed at the 24th and 48thh after transfection. Interference efficiency of the lentivirus expressing cdk4-siRNA was determined by reverse transcriptase-PCR (RT-PCR) and Western blotting in BV-2 cells. Lentivector-mediated RNAi could efficiently down-regulate the expression of the murine cdk4 gene , which provides a potential tool for studying and treating cdk4-related diseases.

Keywords： cyclin-dependent kinase 4     RNA interference     plasmid     lentiviral vector    

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Title Author Date Type Operation

Transport of antibiotic resistance plasmids in porous media and the influence of surfactants

Peipei Chen, Chaoqi Chen, Xiqing Li

Journal Article

The greater roles of indigenous microorganisms in removing nitrobenzene from sediment compared with the

Xiangqun Chi, Yingying Zhang, Daosheng Wang, Feihua Wang, Wei Liang

Journal Article

Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Journal Article

Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination

Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden

Journal Article

Firm innovation system: Paths for enhancing corporate indigenous innovation capability

Jin CHEN, Ximing YIN, Jizhen LI

Journal Article

Genetic diversity and population structure of indigenous chicken breeds in South China

Xunhe HUANG,Jinfeng ZHANG,Danlin HE,Xiquan ZHANG,Fusheng ZHONG,Weina LI,Qingmei ZHENG,Jiebo CHEN,Bingwang DU

Journal Article

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article

Influences and mechanisms of nanofullerene on the horizontal transfer of plasmid-encoded antibiotic resistance

Qingkun Ji, Caihong Zhang, Dan Li

Journal Article

Response of indigenous Cd-tolerant electrochemically active bacteria in MECs toward exotic Cr(VI) based

Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li

Journal Article

Bioleaching of spent Ni-Cd batteries and phylogenetic analysis of an acidophilic strain in acidified sludge

ZHAO Ling, WANG Liang, YANG Dong, ZHU Nanwen

Journal Article

Indigenous and Integrated Innovation Driving the Boom in China's High-Speed Rail Technologies

Ministry of Science and Technology of the PRC

Journal Article

Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Journal Article